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This study explores the application of Signal Amplification by Reversible Exchange (SABRE) to achieve hyperpolarization of unmodified amino acids, specifically using L-[1-13C]-valine as a model. The researchers achieved over 60-fold signal enhancements in nuclear magnetic resonance (NMR) measurements, demonstrating the technique's effectiveness in partially aqueous media. Additionally, they provided preliminary evidence for the method's applicability to other amino acids like glycine, highlighting the potential for broader use in metabolite detection.
Achieving over 60-fold signal enhancements in NMR for unmodified amino acids could revolutionize the detection of low-concentration metabolites.
Hyperpolarization techniques enhance the sensitivity of nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI), enabling detection of low concentration metabolites and dynamic processes. Signal Amplification by Reversible Exchange (SABRE) transfers spin polarization from parahydrogen without permanent chemical modification of the substrate. Here, we demonstrate SABRE-mediated 13C hyperpolarization of unmodified amino acids using L-[1- 13C]-valine as the primary model system and provide preliminary evidence of applicability to glycine. Signal enhancements exceeding 60-fold were observed on a 1.1 T benchtop NMR spectrometer. The dependence of polarization on parahydrogen bubbling time, solvent composition, and substrate-to-catalyst ratio was investigated.
